Characterization of Canine Adenovirus Type 2 Virus Infection Pattern in Canine and Human Cell Lines

Figure 5

Quantitative RT-PCR analysis of CAR mRNA in canine and human cell lines using specific primers. CAR expression levels were normalized by the comparative ΔΔCt method. ΔCt values for each sample were calculated by normalizing to beta-actin as the normalization control. ΔΔCt values were calculated using the mean of ΔCt values of all samples as a reference control. The error bars represent standard deviation. The results presented here are the average of three independent qPCRs.