Characterization of Canine Adenovirus Type 2 Virus Infection Pattern in Canine and Human Cell Lines

Table 1

Percentage of population infected by CAVGFP and Ad5G/L in canine and human cell lines. Cells were transduced by adenovirus CAVGFP and Ad5G/L at 100 multiplicity of infection (MOI 100 virus particles per cell). Percentage of population expressing GFP was analyzed by flow cytometry 48 hours after infection. The results presented here are the average of three independent infection experiments.

			% population
			CAVGFP	Ad5G/L
Canine cell lines	Fetal kidney	FDK	20.8	2.77
		MDCK	85.7	1.49
	Breast tumor	CMT12	4.34	1.11
	Osteosarcoma	CF11	35.9	45.2
		D17	45	50.6
	Melanoma	CML7	6.68	2.15
		CML10	44.5	4.22
	Histiocytic tumor	DH82	20.5	2.63
	Lymphoma	OSW	3.7	0.22
		17–71	14.6	0.89
	Mast cell tumor	MPT1	44	0.23
Human cell line	Prostate cancer	PC3	1.27	1.12
	Ovarian cancer	SKOV3	10.9	3.71
	Liver cancer	HepG2	2.04	31.5
	Colon cancer	LS174	0.42	1.16
		LS180	1.15	1.42
Chinese hamster	Ovary	CHO K1	0.13	0.22