A Unique Trinucleotide-Bloc Mutation-Based Two SARS-CoV-2 Genotypes with Potential Pathogenic Impacts

Figure 4

Impact of the RG > KR mutation on N protein structure. (a) The amino acid mutation at 203–204 position of the N protein changes the size and arrangement of the alpha-helix of the protein as indicated by the red bars. The left panel in the image is the reference N protein, whereas the right panel represents the mutated N protein. Conservations, quality, and consensus have been indicated at the bottom layers of the image. (b) Neighbor-joining clustering based on RG dipeptide after multiple sequence alignments shows seven proteins (green) as the neighbor of the wild type N protein. (c) Clustering based on the mutated KR dipeptide has found only one immediate neighbor (red) to the mutated N protein indicating its rarity. In both cases, the distances of the other branches indicate the different types of dipeptides found in other viruses.

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