Impact of Thermal Pretreatment of Saliva on the RT-PCR Detection of SARS-CoV-2

Table 2

Differences in Ct values and respective values, for factors dilution and heating time, for genes ORF1ab, N, and E, as determined by post hoc Tukey HSD analysis.

Factors		Gene ORF1ab		Gene N		Gene E
		Ct difference	value	Ct difference	value	Ct difference	value
Dilution	1 : 10–1 : 1	0.390	0.4569	0.558	0.5582	0.360	0.2144
	1 : 100–1 : 10	−1.013	0.0206	−1.138	0.0270	−0.566	0.0399
Heating time	10–5	−3.645	3.7 × 10^−6	−4.030	7.2 × 10^−6	−3.095	2.0 × 10^−7
	15–10	−1.957	0.0011	−2.240	0.0014	−1.830	3.61 × 10^−5
	20–15	0.487	0.5560	0.423	0.7609	0.263	0.6736