Bioinorganic Chemistry and Applications

Bioinorganic Chemistry and Applications / 2005 / Article
Special Issue

7th International Symposium on Applied Bioinorganic Chemistry Guanajuato, Mexico, April 2003

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Volume 3 |Article ID 765234 | https://doi.org/10.1155/BCA.2005.109

A. Myari, N. Hadjiliadis, A. Garoufis, "1H NMR Study of the Enantioselective Binding of Λ- and Δ-[Ru(bpy)2(m-bpy-GHK)]Cl2 to the Deoxynucleotide Duplex d(5'-C1G2C3G4A5A6T7T8C9G10C11G12-3')2", Bioinorganic Chemistry and Applications, vol. 3, Article ID 765234, 9 pages, 2005. https://doi.org/10.1155/BCA.2005.109

1H NMR Study of the Enantioselective Binding of Λ- and Δ-[Ru(bpy)2(m-bpy-GHK)]Cl2 to the Deoxynucleotide Duplex d(5'-C1G2C3G4A5A6T7T8C9G10C11G12-3')2

Abstract

The interaction of the diastereomeric complexes Λ- and Δ-[Ru(bpy)2(m-GHK)]Cl2, (GHK = glycine-histidine-lysine) to the deoxynucleotide duplex d(5'-CGCGAATTCGCG-3')2 was studied by means of 1H NMR spectroscopy. The diastereomers interact with the oligonucleotide duplex differently. The Δ-[Ru(bpy)2(m-GHK)]Cl2 is characterized by major groove binding close to the central part of the oligonucleotide, with both the peptide and the bipyridine ligand of the complex involved in the binding. The Λ-[Ru(bpy)2(m-bpy-GHK)]Cl2 binds loosely, approaching the helix from the minor groove. The NMR analysis shows that the peptide (GHK) binding has a determinative role in the interactions of both diastereomers with the oligonucleotide.

Copyright © 2005 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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