The Δ33-35 Mutant 𝛼-Domain Containing 𝛽-Domain-Like M_𝟑S_𝟗 Cluster Exhibits the Function of 𝛼-Domain with M_𝟒S_𝟏𝟏 Cluster in Human Growth Inhibitory Factor

Figure 2

The UV (a) and CD (b) spectra of Cd²⁺-reconstituted hGIF (blue line), the Δ33-35 mutant (green line), the β(MT3)-β(MT3) mutant (black line), and the single β-domain of hGIF (red line). All proteins are dissolved in 10 mM Tris-HCl, 100 mM KCl (pH 8.0).

(a)

(b)