The Δ33-35 Mutant 𝛼-Domain Containing 𝛽-Domain-Like M_𝟑S_𝟗 Cluster Exhibits the Function of 𝛼-Domain with M_𝟒S_𝟏𝟏 Cluster in Human Growth Inhibitory Factor

Figure 3

UV absorbance at 250 nm of pH titration of Cd²⁺-reconstituted hGIF (solid squares), the ∆33-35 mutant (open circles), and the β(MT3)-β(MT3) mutant (solid triangles). All proteins are dissolved in 10 mM Tris-HCl, 100 mM KCl, and pH 8.0.