|ICP-AES inductively coupled plasma atomic emission spectroscopy, ESI-MS electrospray ionization mass spectrometry.|
In all experiments Cd and Cu contents were also measured but their amount was always under detection limits.
Species proposed according to the mass difference between holo-protein and apo-protein. Species in bold are the major components of the preparation.
Experimental molecular masses. Measurements were always performed in duplicate. All corresponding standard deviations were always less than 1%.
Theoretical molecular mass of the corresponding species.
Control experiment: MtnB synthesized in Zn(II)-supplemented medium.