Table 3: Comparison of the Zn-MT complexes recovered from recombinant syntheses in Zn- and nonsupplemented culture media.

MT proteinZn-MT species recovered when synthesized in Zn-supplemented mediaZn-MT species recovered when synthesized in nonsupplemented media Mean Zn : MT molar ratio, when synthesized in Zn-supplemented mediaMean Zn : MT molar ratio, when synthesized in nonsupplemented media Situation in the Zn-/Cu-thionein gradation

mMT1Zn7-mMT1 [7]Zn7-mMT17.3 [7]6.953/16
Crs5Zn6-Crs5 Zn5-Crs5 [10]Zn4-Crs55.7 [10]4.2010/16
MtnAZn4-MtnA [11]Apo-MtnA Zn1-MtnA3.5 [11]1.7014/16
MtnBZn4-MtnB [12]Apo-MtnB3.7 [12]2.0415/16

In this work, only major species identified by ESI-MS are indicated.
In this work, the mean Zn : MT value corresponding to the conventional ICP-AES value is indicated.
The study in [9] includes 16 MT isoforms, classified from the most extreme Zn-thionein to the most extreme Cu-thionein character.