Measuring the Cytochrome Nitrite Reductase Activity—Practical Considerations on the Enzyme Assays
Figure 3
ccNiR specific activities determined with an equivalent electron concentration of the mediators and with a saturating nitrite () and hydroxylamine ()) concentration. Incubation time was 4 minutes, that is, within the period of ammonia production at a linear rate. Dithionite was used as reducing agent. All assays were performed at . Enzyme concentration was 0.7 nM for MV, DQ and PS assays, 7 nM for AQS, and 14 nM for IC.
) and hydroxylamine (
)) concentration. Incubation time was 4 minutes, that is, within the period of ammonia production at a linear rate. Dithionite was used as reducing agent. All assays were performed at . Enzyme concentration was 0.7 nM for MV, DQ and PS assays, 7 nM for AQS, and 14 nM for IC.
