Measuring the Cytochrome Nitrite Reductase Activity—Practical Considerations on the Enzyme Assays
Figure 5
Enzyme activities determined using equivalent electron concentrations (0.16 mM) of MV (■), DQ (♦), PS (), at C, over increasing nitrite concentrations. The solid lines represent the simulations to the Michaelis-Menten equation. Enzyme concentration was 0.6 nM for MV, 1.5 nM for DQ, and 7.4 nM for PS assays.
), at C, over increasing nitrite concentrations. The solid lines represent the simulations to the Michaelis-Menten equation. Enzyme concentration was 0.6 nM for MV, 1.5 nM for DQ, and 7.4 nM for PS assays.
