Effect of Common Buffers and Heterocyclic Ligands on the Binding of Cu(II) at the Multimetal Binding Site in Human Serum Albumin

Figure 2

Titration curves (experimental points, , and fits to the 𝐾_app formula, ) for Cu(II) binding at MBS in four solutions studied: (a) NaCl; (b) Hepes; (c) sodium phosphate; (d) Tris.

(a)

(b)

(c)

(d)