Coal Depolymerising Activity and Haloperoxidase Activity of Mn Peroxidase from Fomes durissimus MTCC-1173

Figure 5

Humic acid depolymerizing activity of the purified enzyme. (a) Increase in absorbance at 360 nm; (b) decrease in absorbance at 460 nm. The reaction solution 1 mL contained “200 μg of humic acid, 5 μg of the enzyme (4 IU/mg), 40 μM H₂O₂, 100 μM MnSO₄ in 20 mM of buffers pH 4.5.” Sodium succinate buffer (⚫), sodium lactate buffer (♦), and sodium malonate buffer (▲).

(a)

(b)