Research Article
Coal Depolymerising Activity and Haloperoxidase Activity of Mn Peroxidase from Fomes durissimus MTCC-1173
Table 1
Purification chart for Mn peroxidase from the culture filtrate of the fungal strain Fomes durissimus MTCC-1173.
| | S. no. | Steps | Total vol.
(mL) | Protein
(mg/mL) | activity (IU/mL) | Specific activity (IU/mg) | Total protein (mg) | Total activity (IU) | Purification Fold | % Recovery |
| | (1) | Crude enzyme | 800 | 0.50 | 0.95 | 1.90 | 400.00 | 760.00 | 1.00 | 100.00 | | (2) | Concentrated enzyme | 10 | 0.85 | 3.14 | 3.69 | 8.50 | 31.40 | 1.94 | 4.13 | | (3) | Dialysed enzyme | 12 | 0.80 | 2.90 | 3.62 | 9.60 | 34.80 | 1.91 | 4.57 | | (4) | DEAE | 35 | 0.15 | 1.40 | 9.33 | 5.25 | 49.00 | 4.91 | 6.44 |
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