Coal Depolymerising Activity and Haloperoxidase Activity of Mn Peroxidase from Fomes durissimus MTCC-1173

Table 2

𝐾_𝑚, 𝑘_cat, and 𝑘_cat/𝐾_𝑚 values for the purified Mn peroxidase in presence of different chelating agents.

Chelating agents	Mn peroxidase of F. durissimus			Mn peroxidase of P. chrysosporium [21]
	𝐾𝑚 (μM)	𝑘cat (s^−1)	𝑘cat/𝐾𝑚 (M^−1 s^−1)	𝐾𝑚 (μM)	𝑘cat (s^−1)	𝑘cat/𝐾𝑚 (M^−1 s^−1)
H2O2	32.0*	14.0*	0.44 × 10^6*	39.0	—	6.3×10^6
Oxalate	11.9	8.4	0.71×10^6	13.0	308.0	2.4×10^7
Lactate	59.0	22.4	0.38×10^6	41.0	211.0	5.1×10^6
Malonate	47.0	30.8	0.65×10^6	18.0	220.0	1.2×10^7

*The values for H₂O₂ were in lactate buffer.