Role of Superoxide Anions in the Redox Changes Affecting the Physiologically Occurring Cu(I)-Glutathione Complex
Figure 3
Effect of EDTA on the recovery of GSSG from the Cu(II)-GSSG complex assayed by the NADPH/glutathione reductase assay. NADPH (0.2 mM), glutathione reductase (2 U/mL), and EDTA (2 mM) were added to a solution containing GSSG (36 μM) or a preformed Cu(II)-GSSG complex (36 μM). The decrease in O was registered and the results expressed as μM GSSG concentration. The asterisk on the bars indicate that such values are statistically different, at the level of a , from all other values.
