Role of Superoxide Anions in the Redox Changes Affecting the Physiologically Occurring Cu(I)-Glutathione Complex
Figure 5
Recovery of GSSG from a Tempol-treated Cu(I)-[GSH]2 complex using the EDTA-modified NADPH/glutathione reductase assay. GSSG was assessed applying the NADPH/glutathione reductase assay to solutions containing pure GSSG (18 or 54 μM), the Cu(I)-[GSH]2 complex (36 μM) or a Tempol-treated Cu(I)-[GSH]2 complex (36 μM). The assays were run both in the presence or in the absence of EDTA (2 mM). Results are expressed as μM GSSG concentration. The asterisk on the bars indicates that such values are statistically different, at the level of a , from all other values.
