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Bioinorganic Chemistry and Applications
Volume 2014 (2014), Article ID 581890, 10 pages
Research Article

In Vitro Antibacterial Activity and Mechanism of Silver Nanoparticles against Foodborne Pathogens

1Department of Biochemistry, Adhiparasakthi College of Arts and Science, Kalavai, Vellore District 632506, Tamil Nadu, India
2Environmental Nanotechnology Division, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412, Tamil Nadu, India

Received 31 May 2014; Accepted 27 August 2014; Published 17 September 2014

Academic Editor: Nick Katsaros

Copyright © 2014 S. Rajeshkumar and C. Malarkodi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Biosynthesis of silver nanoparticles using Planomicrobium sp. and to explore the antibacterial activity against food borne pathogenic bacteria Bacillus subtilis, (3053) Klebsiella planticola (2727) Klebsiella pneumoniae (MAA) Serratia nematodiphila (CAA) and Escherichia coli. In the current studies, 1 mM of silver nitrate was added into 100 mL of Planomicrobium sp. culture supernatant. The bioreduction of pure AgNO3 was characterized by UV-visible spectroscopy, X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), energy-dispersive analysis (EDS), transmission electron microscopy (TEM), and Fourier transform infrared (FT-IR) analysis. The formation of silver nanoparticles was confirmed by the presence of an absorption peak at 400 nm using UV-visible spectrophotometry. The morphology and size of the silver nanoparticles was monitored by TEM and SEM. Crystal structure was obtained by carrying out X-ray diffraction studies and it showed face centered cubic (FCC) structure. The bactericidal effect of silver nanoparticles was compared based on diameter of inhibition zone in well method. Bacterial sensitivity to nanoparticles a key factor in manufacture the suitable for long life application in food packaging and food safety. Food safety is a worldwide health goal and the food borne diseases get a main disaster on health. Therefore, controlling of bacterial pathogens in food is credit of harms associated to health and safety.