Protonation Equilibria of Biologically Active Ligands in Mixed Aqueous Organic Solvents

Table 13

Effect of temperature and thermodynamic parameters of protonation equilibria of inosine and IMP^a.

System	(°C)				Equilibrium	(kJ mol^−1)	(JK^−1 mol^−1)	(kJ mol^−1)
Inosine	15	1	1	8.77	Inosine	−35.0	46.3	−48.8
IMP	15	1	1	9.33	(1) L^− + H^+ LH
		1	2	15.28
Inosine	20	1	1	8.67
IMP	20	1	1	9.17
		1	2	15.17
Inosine	25	1	1	8.55	IMP	−33.0	64.6	−52.2
IMP	25	1	1	9.09	(2) L^3−+ H^+ LH^2−	0.32	114.6	−33.8
		1	2	15.07	(3) LH^2− + H^+
Inosine	30	1	1	8.44
IMP	30	1	1	8.95
		1	2	14.99

and are stoichiometric coefficients corresponding to ligand and H⁺, respectively; ^bdata taken from reference [83]; L denotes inosine and inosine 5′-monophosphate.