Reversible Dissociation and Ligand-Glutathione Exchange Reaction in Binuclear Cationic Tetranitrosyl Iron Complex with Penicillamine
Figure 5
Kinetics of change of absorption spectrum of (II) ( M): spectra were registered at 30 s , 5 min after start of reaction. Spectra 3–16 were registered further with interval 20 min. Spectra 17–22 were registered at 6.5 (17), 10.1 (18), 13 (19), 17.5 (20), 20 (21), and 24 (22) h after start of reaction. Conditions of reaction: 25°C, solvent is 0.05 M Tris-HCl buffer, and pH 7.0. Spectra 1–22 have 2 maxima: = 315 nm and = 365 nm; is equal to M−1·cm−1. The inset shows kinetics of (II) ( M) decomposition in 0.05 M Tris-HCl-buffer pH 7.0 at 25°C (for the experimental data shown on this figure). Circles are experimental data. Approximation (theoretical curve) was made by means of . (II) is the complex .
