Reversible Dissociation and Ligand-Glutathione Exchange Reaction in Binuclear Cationic Tetranitrosyl Iron Complex with Penicillamine
Figure 8
Kinetics of absorption changes in the interaction of (I) ( M) with GSH (10−3 M): spectra were registered at 45 s , 5 min , and 20 min after start of reaction. Spectra 4–18 were registered further with interval 20 min. Spectra 19–28 were registered at 7.3 (19), 9.4 (20), 11.8 (21), 13.8 (22), 16.3 (23), 19.5 (24), 21.5 (25), 22.8 (26), 23 (27), and 24 (28) h after start of reaction. Conditions of reaction: 25°C, solvent is 0.05 M Tris-HCl buffer, pH 7.0. Spectra 1–28 have 2 maxima: = 315 nm and = 367 nm. The inset shows kinetics of (II) accumulation at interaction of (I) with GSH on the base of the experimental data shown on this figure. Circles are the experimental data. Approximation (theoretical curve) was made by means of . (I) is the complex , and (II) is the complex .
