Arsenic Primes Human Bone Marrow CD34+ Cells for Erythroid Differentiation

Table 1

Effects of ATO pretreatment on colony forming activities of human BM CD34+ cells.

	Control	ATO
Total CFU (per 10^3 cells)	58.0 ± 5.7	69.5 ± 5.6	0.2
CFU-E (%)	4.1 ± 0.9	14.9 ± 3.8	0.03
BFU-E (%)	34.4 ± 2.5	48.9 ± 3.7	0.02
Total E (%)	38.5 ± 2.3	63.3 ± 5.6	0.007
CFU-GM (%)	59.7 ± 2.3	33.3 ± 5.6	0.005
CFU-GEMM (%)	1.8 ± 0.2	3.3 ± 0.5	0.03

Bone marrow CD34+ cells primed with 0.5 μM were seeded in 6-well plates (1000 cells per well) and colonies counted on day 16. CFU: colony forming unit; BFU: burst forming unit; E: erythroid; GM: granulocyte macrophage; GEMM: granulocyte, erythroid, macrophage, and megakaryocyte. Unpaired -test was performed for comparison of the mean data (mean ± SEM, experiments in quadruplicate).