Gamma Radiation-Induced Damage in the Zinc Finger of the Transcription Factor IIIA

Figure 5

Restoration of the binding capacity of irradiated transcription factor IIIA (TFIIIA) with the internal control region (ICR) by DL-dithiothreitol (DTT) and ZnCl₂ solutions. (a) Band of binding of gamma-irradiated TFIIIA (1000 Gy) with the ICR after irradiated TFIIIA was incubated with 0.5 mM of DTT and 50 µM of ZnCl₂. (b) Integral optical density (IOD) of the binding after irradiated TFIIIA was incubated with ZnCl₂ (50 µM), DTT (0.5 mM), and DTT (0.5 mM) and ZnCl₂ (50 µM), respectively. Z: 50 µM of ZnCl₂. D: 0.5 mM of DTT.

(a)

(b)