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Bioinorganic Chemistry and Applications
Volume 2016, Article ID 4121960, 7 pages
http://dx.doi.org/10.1155/2016/4121960
Research Article

The Role of Metals in the Reaction Catalyzed by Metal-Ion-Independent Bacillary RNase

1Institute of Fundamental Medicine and Biology, Kazan Federal University, Kremlevskaya Str. 18, Kazan 420008, Russia
2Institute of Physics, Kazan Federal University, Kremlevskaya Str. 16a, Kazan 420008, Russia
3Institute for Anatomy and Cell Biology, Justus Liebig University Giessen, Aulweg 123, 35385 Giessen, Germany

Received 25 August 2016; Revised 27 October 2016; Accepted 2 November 2016

Academic Editor: Spyros P. Perlepes

Copyright © 2016 Yulia Sokurenko et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Extracellular enzymes of intestinal microbiota are the key agents that affect functional activity of the body as they directly interact with epithelial and immune cells. Several species of the Bacillus genus, like Bacillus pumilus, a common producer of extracellular RNase binase, can populate the intestinal microbiome as a colonizing organism. Without involving metal ions as cofactors, binase depolymerizes RNA by cleaving the 3′,5′-phosphodiester bond and generates 2′,3′-cyclic guanosine phosphates in the first stage of a catalytic reaction. Maintained in the reaction mixture for more than one hour, such messengers can affect the human intestinal microflora and the human body. In the present study, we found that the rate of 2′,3′-cGMP was growing in the presence of transition metals that stabilized the RNA structure. At the same time, transition metal ions only marginally reduced the amount of 2′,3′-cGMP, blocking binase recognition sites of guanine at N7 of nucleophilic purine bases.