Research Article
The Role of Metals in the Reaction Catalyzed by Metal-Ion-Independent Bacillary RNase
Figure 1
Regions of 1H NMR titration (700 MHz) for binase with increasing amount of (a) MgCl2 from 0 to 1 mM, (b) ZnCl2 from 0 to 1 mM, and (c) MnCl2 from 0 to 3 mM. Binase concentration was 1 mg/mL. NMR-spectra were recorded at 298 K, pH 5.5.
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