The Role of Metals in the Reaction Catalyzed by Metal-Ion-Independent Bacillary RNase

Figure 2

Regions of ¹H NMR titration (500 MHz) for RNA with increasing amount of (a) MgCl₂, (b) СаCl₂, (c) ZnCl₂, and (d) MnCl₂ from 0 to 5 mM. RNA concentration was 1 mg/mL. NMR-spectra were recorded at 298 K, pH 5.5.

(a)

(b)

(c)

(d)