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Bioinorganic Chemistry and Applications
Volume 2017, Article ID 1064918, 6 pages
https://doi.org/10.1155/2017/1064918
Research Article

Antimicrobial Properties of Copper Nanoparticles and Amino Acid Chelated Copper Nanoparticles Produced by Using a Soya Extract

1Doctorado Institucional en Ingeniería y Ciencia de Materiales, Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, Mexico
2Facultad de Ciencias, Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, Mexico
3Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, Mexico
4Departamento Físico Matemático, Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, Mexico
5Coordinación para la Innovación y Aplicación de la Ciencia y Tecnología, Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, Mexico

Correspondence should be addressed to M. E. Compeán Jasso; moc.liamg@naepmoc.ahtram

Received 15 November 2016; Accepted 10 January 2017; Published 13 February 2017

Academic Editor: Claudio Pettinari

Copyright © 2017 I. DeAlba-Montero et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This paper reports a comparison of the antibacterial properties of copper-amino acids chelates and copper nanoparticles against Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis. These copper-amino acids chelates were synthesized by using a soybean aqueous extract and copper nanoparticles were produced using as a starting material the copper-amino acids chelates species. The antibacterial activity of the samples was evaluated by using the standard microdilution method (CLSI M100-S25 January 2015). In the antibacterial activity assays copper ions and copper-EDTA chelates were included as references, so that copper-amino acids chelates can be particularly suitable for acting as an antibacterial agent, so they are excellent candidates for specific applications. Additionally, to confirm the antimicrobial mechanism on bacterial cells, MTT assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) was carried out. A significant enhanced antimicrobial activity and a specific strain were found for copper chelates over E. faecalis. Its results would eventually lead to better utilization of copper-amino acids chelate for specific application where copper nanoparticles can be not used.