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Bioinorganic Chemistry and Applications
Volume 2017, Article ID 2562780, 9 pages
https://doi.org/10.1155/2017/2562780
Research Article

Synthesis, Characterization, Cytotoxic Activity, and Interactions with CT-DNA and BSA of Cationic Ruthenium(II) Complexes Containing Dppm and Quinoline Carboxylates

1Instituto de Química, Universidade Federal de Uberlândia, 38400-902 Uberlândia, MG, Brazil
2Departamento de Química, Universidade Federal de São Carlos, 13565-905 São Carlos, SP, Brazil
3Universidade de Franca, 14404-600 Franca, SP, Brazil

Correspondence should be addressed to Gustavo Von Poelhsitz; rb.ufu@ztishleopovatsug

Received 6 March 2017; Revised 30 May 2017; Accepted 13 June 2017; Published 26 July 2017

Academic Editor: Claudio Pettinari

Copyright © 2017 Edinaldo N. da Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The complexes cis-[Ru(quin)(dppm)2]PF6 and cis-[Ru(kynu)(dppm)2]PF6 (quin = quinaldate; kynu = kynurenate; dppm = bis(diphenylphosphino)methane) were prepared and characterized by elemental analysis, electronic, FTIR, 1H, and 31P NMR spectroscopies. Characterization data were consistent with a cis arrangement for the dppm ligands and a bidentate coordination through carboxylate oxygens of the quin and kynu anions. These complexes were not able to intercalate CT-DNA as shown by circular dichroism spectroscopy. On the other hand, bovine serum albumin (BSA) binding constants and thermodynamic parameters suggest spontaneous interactions with this protein by hydrogen bonds and van der Waals forces. Cytotoxicity assays were carried out on a panel of human cancer cell lines including HepG2, MCF-7, and MO59J and one normal cell line GM07492A. In general, the new ruthenium(II) complexes displayed a moderate to high cytotoxicity in all the assayed cell lines with IC50 ranging from 10.1 to 36 µM and were more cytotoxic than the precursor cis-[RuCl2(dppm)2]. The cis-[Ru(quin)(dppm)2]PF6 were two to three times more active than the reference metallodrug cisplatin in the MCF-7 and MO59J cell lines.