Ir-6: A Novel Iridium (III) Organometallic Derivative for Inhibition of Human Platelet Activation

Figure 5

Inhibitory effects of Ir-6 on the phosphorylation of p38 MAPK, ERK2, and JNK1 in human platelets. The washed platelets (1.2 × 10⁹ cells/mL) were preincubated with the solvent control (0.1% DMSO), SB203580 (10 µM), PD98059 (20 µM), SP600125 (10 µM), or Ir-6 (10 or 20 µM); subsequently, collagen (1 g/mL) was added to trigger platelet activation. The platelets were collected, and their subcellular extracts were then analyzed to determine the levels of phosphorylation of (a) p38 MAPK, (b) ERK2, and (c) JNK1. Data are presented as the mean ± standard error of the mean (). , , and , compared with the resting control; ^### and ^###, compared with the DMSO-treated group. The profiles of left panels are representative of four independent experiments.

(a)

(b)

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