High Throughput Approaches to Unravel the Mechanism of Action of a New Vanadium-Based Compound against Trypanosoma cruzi

Figure 3

Cell death mechanism and morphological analysis of V^IVO(5Brsal)(aminophen) treated T. cruzi epimastigotes. (a) Flow cytometry analysis of parasites, untreated (control) and treated with V^IVO(5Brsal)(aminophen) for 24 h at the indicated concentrations (1x IC₅₀, 5x IC₅₀, and 10x IC₅₀), labeled with annexin V and propidium iodide. Dot plots represent unlabeled parasites (lower left square), early apoptotic annexin V labeled parasites (upper left square), and late apoptotic/necrotic annexin V/propidium iodide double labeled parasites (upper right square). (b) Flow cytometry analysis of parasites, untreated (black line) and incubated with the vanadium-based compound for 24 h at 1x IC₅₀ (gray line), 5x IC₅₀ (solid filled black line), and 10x IC₅₀ (solid filled gray line), labeled with Calcein AM (left panel) and with propidium iodide (right panel). (c) Scanning electron microscopy image of a representative control untreated parasite (left image) and epimastigotes treated with 5x IC₅₀ of V^IVO(5Brsal)(aminophen) for 6 h. Images were obtained with a Philips XL30 scanning electron microscope (magnification of 10,000 x.)

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