High Throughput Approaches to Unravel the Mechanism of Action of a New Vanadium-Based Compound against Trypanosoma cruzi

Figure 5

Uptake and macromolecule association of V^IVO(5Brsal)(aminophen) in T. cruzi epimastigotes. (a) Amount of vanadium strongly bound and/or uptaken by compound-treated parasites. Parasites were incubated with 3.76 μM (1x IC₅₀), 11.28 μM (5x IC₅₀), and 37.6 μM (10x IC₅₀) of V^IVO(5Brsal)(aminophen), and incorporated vanadium was determined through electrothermal atomic absorption spectrometry. (b) Percentage of total vanadium associated with DNA, RNA. (c) Determination of total vanadium associated with soluble and insoluble fraction. Vanadium amounts were determined through electrothermal atomic absorption spectrometry after 6 h of incubation with 5x IC₅₀. The average of two independent experiments is presented with its associated standard deviation.

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