Synthesis and Characterization of Green Zinc Oxide Nanoparticles with Antiproliferative Effects through Apoptosis Induction and MicroRNA Modulation in Breast Cancer Cells
Table 3
The t-test was performed for the fold change values for caspase-3 and caspase-8 gene expression analysis in ZnO-NP-treated cells.
Variable
Caspase-3 expression (fold change) based on β-actin
value
Caspase-3 expression (fold change) based on GAPDH
value
HFF
Control
1.00 ± 0.13
0.020
1.00 ± 0.18
0.819
Treatment
0.61 ± 0.11
0.952 ± 0.21
MCF7
Control
1.00 ± 0.22
0.239
1.00 ± 0.16
0.821
Treatment
1.16 ± 0.23
0.959 ± 0.15
Variable
Caspase-8 expression (fold change) based on β-actin
p value
Caspase-8 expression (fold change) based on GAPDH
p value
HFF
Control
1.00 ± 0.12
0.020
1.00 ± 0.24
0.016
Treatment
1.65 ± 0.20
2.56 ± 0.32
MCF7
Control
1.00 ± 0.14
0.1
1.00 ± 0.16
0.41
Treatment
1.61 ± 0.15
1.33 ± 0.15
Abbreviations: MCF7: Michigan Cancer Foundation-7, HFF: human foreskin fibroblasts, miR: microRNA, and GAPDH: glyceraldehyde 3‐phosphate dehydrogenase.
