Ru(II) Complexes Bearing O, O-Chelated Ligands Induced Apoptosis in A549 Cells through the Mitochondrial Apoptotic Pathway

Figure 3

(a) A549 cells stained with Hoechst 33342 after treatment of Ru(II) complexes at 20 μΜ for 24 h. (b) Apoptosis in A549 cells was detected by Annexin V/PI assay after coincubation with different concentrations of Ru(II) complexes for 24 h (I2: late apoptotic or necrotic cells, I3: living cells, and I4: early apoptotic cells). (c) The expression levels of caspase-3/8, PARP, cleaved caspase-3/8/9, and PARP were evaluated in a concentration-dependent manner with Ru-1 treatment for 24 h.

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