Research Article
Complexed Crystal Structure of Saccharomyces cerevisiae Dihydroorotase with Inhibitor 5-Fluoroorotate Reveals a New Binding Mode
Table 2
Binding parameters of ScDHOase to 5-FOA.
| | DHOase | λmax (nm) | λem shift (nm) | Quenching (%) | Kd value (μM) |
| | ScDHOase | From 324 to 335.5 | 11.5 | 78.2 | 83.8 ± 1.5 | | ScDHOase-R18A | From 324.5 to 334.5 | 10.0 | 75.9 | 143.6 ± 2.1 | | ScDHOase-T106A | From 328 to 333.5 | 5.5 | 64.6 | 114.8 ± 3.7 |
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