GE11 Peptide Conjugated Liposomes for EGFR-Targeted and Chemophotothermal Combined Anticancer Therapy

Figure 4

(a) Immunocytochemical staining and (b) protein level of EGFR expression in LO2 cells, HeLa cells, and A549 cells. Scale bar = 50 µm. (c) FL images of A549 cells after 4 h of incubation with free ICG, ICG-LPs, GE11-ICG-LPs, and GE11-ICG-LPs with free GE11 peptide or anti-EGFR antibody blocking. DAPI (blue) counterstains cell nuclei. Scale bar = 50 µm. (d) The semiquantitative analysis of fluorescence intensity in (c) was determined by ‘Image J’ software. Data are expressed as mean ± SD (n = 3). (e) FL images of A549 cells incubated with GE11-CUR/ICG-LPs after laser irradiation (1 W·cm⁻²) for different times. Scale bar = 50 µm.

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