Chronological Age Estimation of Male Occipital Bone Based on FTIR and Raman Microspectroscopy

Figure 2

Four outcomes at 6 age stages were evaluated by FTIR and Raman and displayed by box and mean line plots. (a) FTIR mineral/matrix ratio (900–1200 cm⁻¹/1592–1712 cm⁻¹). (b) FTIR carbonate/phosphate ratio (850–890 cm⁻¹/900–1200 cm⁻¹). (c) FTIR crystallinity (1030 cm⁻¹/1020 cm⁻¹). (d) FTIR collagen maturity (1660 cm⁻¹/1690 cm⁻¹). (e) Raman mineral/matrix ratio (410–460 cm⁻¹/1215–1300 cm⁻¹). (f) Raman carbonate/phosphate ratio (1050–1100 cm⁻¹/930–980 cm⁻¹). (g) Raman crystallinity (1/FWHM PO₄³⁻ν₁). (h) Raman collagen maturity (1660 cm⁻¹/1690 cm⁻¹). Significant differences are indicated, , (SNK or Mann–Whitney U-test). Pearson correlation coefficients R between the techniques are indicated in (a)–(d).

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