Plant Extract Based on Deep Eutectic Solvent-Mediated Biosynthesis of Silver Nanoparticles: Cytotoxicity and Antibacterial Effects

Table 3

Characterization of the main compounds of the grape pomace extract using their spectral characteristics in HPLC-DAD (Rt, λmax, and MS2).

Peak	Rt/min	Mode of ionization [M+H]^+/[M−H]^−	Fragments MS2	UV-Vis max	Compound	Percentage
						Solvent
						DES-1	DES-2	Water
Anthocyanins
9	15.2	465/—	303	277, 560	Delphinidin 3-O-glucoside	5.1	4.7	2.4
10	16.4	595/—	449, 287	280, 540	Cyanidin-3-O-glucoside	2.7	2.1	1.5
11	16.6	—/493	311	278, 528	Malvidin 3-O-glucoside	7.1	6.4	5.7
12	17.1	479/—	317	278, 527	Petunidin 3-O-glucoside	6.5	6.0	4.7
Phenolic acids
1	1.8	—/169	125, 107, 97, 79	325	Gallic acid	10.8	10.4	11.2
2	4.7	—/153	109	325	Protocatechuic acid	5.2	4.1	4.9
3	5.0	—/137	93	325	p-Hydroxybenzoic acid	3.2	2.8	3.0
4	5.2	—/295	163	325	Coutaric acid	2.7	1.8	2.1
5	6.4	—/179	135	325	Caffeic acid	8.7	6.5	12.8
6	7.3	—/289	245	325	Epicatechin	10.2	8.1	5.2
7	7.7	—/197	153/182	325	Syringic acid	4.2	4.0	5.8
8	8.0	—/163	119	325	p-Coumaric acid	3.1	2.7	3.0
Anthoxanthins and stilbenes
13	20.3	—/289	245	276	(+)-Catechin	9.5	8.1	5.6
14	20.5	—/463	300, 255, 151	354	Quercetin-3-O-glucoside	1.8	1.5	1.4
15	23.8	—/609	300	354	Quercetin-3-O-rutinoside	4.8	4.0	3.2
16	24.5	—/447	284, 255, 227	354	Kaempferol-3-O-glucoside	3.5	3.0	2.7
17	25.0	—/301	301, 151	254	Quercetin	4.3	3.7	2.5

Verified with pure standard. Rt: retention time.