Table of Contents
Bone Marrow Research
Volume 2012, Article ID 539825, 7 pages
Clinical Study

The Presence of Anti-HLA Antibodies before and after Allogeneic Hematopoietic Stem Cells Transplantation from HLA-Mismatched Unrelated Donors

1Department of Hematology and BMT, Medical University of Silesia, Dabrowskiego 25, 40-032 Katowice, Poland
2HLA and Immunogenetics Laboratory, Regional Blood Center, Raciborska 15, 40-074 Katowice, Poland
3Lower Silesian Center for Cellular Transplantation with National Bone Marrow Donor Registry, Grabiszynska 105, 53-439 Wroclaw, Poland

Received 26 June 2012; Revised 17 September 2012; Accepted 18 September 2012

Academic Editor: Bronwen Shaw

Copyright © 2012 Anna Koclega et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Although anti-human leukocyte antigen antibodies (anti-HLA Abs) are important factors responsible for graft rejection in solid organ transplantation and play a role in post-transfusion complications, their role in allogeneic hematopoietic stem cell transplantation (allo-HSCT) has not been finally defined. Enormous polymorphism of HLA-genes, their immunogenicity and heterogeneity of antibodies, as well as the growing number of allo-HSCTs from partially HLA-mismatched donors, increase the probability that anti-HLA antibodies could be important factors responsible for the treatment outcomes. We have examined the incidence of anti-HLA antibodies in a group of 30 allo-HSCT recipients from HLA-mismatched unrelated donors. Anti-HLA Abs were identified in sera collected before and after allo-HSCT. We have used automated DynaChip assay utilizing microchips bearing purified class I and II HLA antigens for detection of anti-HLA Abs. We have detected anit-HLA antibodies against HLA-A, B, C, DR, DQ and DP, but no donor or recipient-specific anti-HLA Abs were detected in the studied group. The preliminary results indicate that anti-HLA antibodies are present before and after allo-HSCT in HLA-mismatched recipients.