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Journal of Biomedicine and Biotechnology
Volume 1, Issue 2, Pages 79-84
Research article

Response of Escherichia Coli Containing Mycobacterial Carotene Genes to UV Radiation

1Biotechnology Laboratory, Sidi Mohammed Ben Abdellah University, FST, P.O. Box 2202, Fez, Morocco
2Tuberculosis and Mycobacteria unit, Pasteur Institut, P.O. Box 484, Pointe a Pitre, Guadeloupe

Copyright © 2001 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurumA +, was partially digested by restriction endonucleases and generated fragments were cloned. After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51). E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.