BioMed Research International

BioMed Research International / 2003 / Article
Special Issue

Proteomics in Health and Disease — Part II

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Research article | Open Access

Volume 2003 |Article ID 291092 | https://doi.org/10.1155/S1110724303209268

Weiping Shao, Zhimin Zhou, Isabelle Laroche, Hong Lu, Qiuling Zong, Dhavalkumar D. Patel, Stephen Kingsmore, Steven P. Piccoli, "Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling", BioMed Research International, vol. 2003, Article ID 291092, 9 pages, 2003. https://doi.org/10.1155/S1110724303209268

Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling

Received13 Aug 2002
Accepted10 Dec 2002

Abstract

Protein microarray-based approaches are increasingly being used in research and clinical applications to either profile the expression of proteins or screen molecular interactions. The development of high-throughput, sensitive, convenient, and cost-effective formats for detecting proteins is a necessity for the effective advancement of understanding disease processes. In this paper, we describe the generation of highly multiplexed, antibody-based, specific, and sensitive protein microarrays coupled with rolling-circle signal amplification (RCA) technology. A total of 150 cytokines were simultaneously detected in an RCA sandwich immunoassay format. Greater than half of these proteins have detection sensitivities in the pg/ml range. The validation of antibody microarray with human serum indicated that RCA-based protein microarrays are a powerful tool for high-throughput analysis of protein expression and molecular diagnostics.

Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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