BioMed Research International

BioMed Research International / 2004 / Article

Research article | Open Access

Volume 2004 |Article ID 521320 | https://doi.org/10.1155/S1110724304308077

Vanesa G. Pannunzio, Hilda I. Burgos, Manuel Alonso, James R. Mattoon, Eugenia H. Ramos, Carlos A. Stella, "A Simple Chemical Method for Rendering Wild-Type Yeast Permeable to Brefeldin A That Does Not Require the Presence of an erg6 Mutation", BioMed Research International, vol. 2004, Article ID 521320, 6 pages, 2004. https://doi.org/10.1155/S1110724304308077

A Simple Chemical Method for Rendering Wild-Type Yeast Permeable to Brefeldin A That Does Not Require the Presence of an erg6 Mutation

Received10 Sep 2003
Revised30 Dec 2003
Accepted29 Jan 2004

Abstract

The present work aims to develop a growth medium to render a wild-type strain of Saccharomyces cerevisiae permeable to the antifungal drug Brefeldin A. In the current study, a synthetic medium containing 0.1% L-proline and supplemented with 3.0×103% SDS is employed. When Brefeldin A is added to this medium, a wild-type strain shows increased growth sensitivity and a diminished transport of the amino acid L-leucine. Since Brefeldin A exerts its effect on the endoplasmic reticulum and the Golgi apparatus, the medium permits the study of the drug effect on the intracellular traffic of L-leucine permeases.

Copyright © 2004 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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