BioMed Research International

BioMed Research International / 2004 / Article

Review article | Open Access

Volume 2004 |Article ID 686401 | https://doi.org/10.1155/S1110724304308016

Renu Tuteja, Narendra Tuteja, "Serial Analysis of Gene Expression: Applications in Malaria Parasite, Yeast, Plant, and Animal Studies", BioMed Research International, vol. 2004, Article ID 686401, 7 pages, 2004. https://doi.org/10.1155/S1110724304308016

Serial Analysis of Gene Expression: Applications in Malaria Parasite, Yeast, Plant, and Animal Studies

Received07 Aug 2003
Revised13 Nov 2003
Accepted13 Nov 2003

Abstract

The serial analysis of gene expression (SAGE) method is based on the isolation of unique sequence tags from individual transcripts and concatenation of tags serially into long DNA molecules. SAGE is an innovative technique that offers the potential of cataloging both the identity and relative frequencies of mRNA transcripts in a given RNA preparation. It can quantify low-abundance transcripts and reliably detect relatively small differences in transcript abundance between cell populations. SAGE data can be used to complement studies in cases where other gene expression methods may be more convenient or efficient. SAGE can be used in a wide variety of applications to identify disease-related genes, to analyze the effect of drugs on tissues, and to provide insights into the disease pathways. The most important application of SAGE is the identification of differentially expressed genes. In this review, we describe various applications of this powerful technology in malarial parasite, yeast, plant, and animal systems.

Copyright © 2004 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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