Abstract
We have investigated calcium-induced secretion in human
neutrophils, using a whole-cell patch-clamp technique.
Mobilization of subcellular granules to the cell membrane was
followed as the change in membrane capacitance (
We have investigated calcium-induced secretion in human
neutrophils, using a whole-cell patch-clamp technique.
Mobilization of subcellular granules to the cell membrane was
followed as the change in membrane capacitance (
N D Burg and M H Pillinger, “The neutrophil: function and regulation in innate and humoral immunity,” Clinical Immunology, vol. 99, no. 1, pp. 7–17, 2001.
View at: Google ScholarG Berton, “Degranulation,” in Inflammation: Basic Principles and Clinical Correlates, J I Gallin and R Snyderman, Eds., pp. 703–719, Lippincott Williams & Wilkins, Philadelphia, Pa, 1999.
View at: Google ScholarN Borregaard and J B Cowland, “Granules of the human neutrophilic polymorphonuclear leukocyte,” Blood, vol. 89, no. 10, pp. 3503–3521, 1997.
View at: Google ScholarH Sengelov, P Follin, L Kjeldsen, K Lollike, C Dahlgren, and N Borregaard, “Mobilization of granules and secretory vesicles during in vivo exudation of human neutrophils,” Journal of Immunology, vol. 154, no. 8, pp. 4157–4165, 1995.
View at: Google ScholarT Sollner, S W Whiteheart, M Brunner et al., “SNAP receptors implicated in vesicle targeting and fusion,” Nature, vol. 362, no. 6418, pp. 318–324, 1993.
View at: Google ScholarY A Chen and R H Scheller, “SNARE-mediated membrane fusion,” Nature Reviews Molecular Cell Biology, vol. 2, no. 2, pp. 98–106, 2001.
View at: Google ScholarJ H Brumell, A Volchuk, H Sengelov et al., “Subcellular distribution of docking/fusion proteins in neutrophils, secretory cells with multiple exocytic compartments,” Journal of Immunology, vol. 155, no. 12, pp. 5750–5759, 1995.
View at: Google ScholarB Martin-Martin, S M Nabokina, J Blasi, P A Lazo, and F Mollinedo, “Involvement of SNAP-23 and syntaxin 6 in human neutrophil exocytosis,” Blood, vol. 96, no. 7, pp. 2574–2583, 2000.
View at: Google ScholarF Mollinedo, B Martin-Martin, J Calafat, S M Nabokina, and P A Lazo, “Role of vesicle-associated membrane protein-2, through Q-soluble N-ethylmaleimide-sensitive factor attachment protein receptor/R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor interaction, in the exocytosis of specific and tertiary granules of human neutrophils,” Journal of Immunology, vol. 170, no. 2, pp. 1034–1042, 2003.
View at: Google ScholarR D Burgoyne and A Morgan, “ and secretory-vesicle dynamics,” Trends in Neurosciences, vol. 18, no. 4, pp. 191–196, 1995.
View at: Google ScholarN W Andrews, “Regulated secretion of conventional lysosomes,” Trends in Cell Biology, vol. 10, no. 8, pp. 316–321, 2000.
View at: Google ScholarP D Lew, A Monod, K-H Krause, F A Waldvogel, T J Biden, and W Schlegel, “The role of cytosolic free calcium in the generation of inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate in HL-60 cells. Differential effects of chemotactic peptide receptor stimulation at distinct levels,” Journal of Biological Chemistry, vol. 261, no. 28, pp. 13121–13127, 1986.
View at: Google ScholarM M Barrowman, S Cockcroft, and B D Gomperts, “Differential control of azurophilic and specific granule exocytosis in Sendai-virus-permeabilized rabbit neutrophils,” Journal of Physiology, vol. 383, pp. 115–124, 1987.
View at: Google ScholarN Borregaard, K Lollike, L Kjeldsen et al., “Human neutrophil granules and secretory vesicles,” European Journal of Haematology, vol. 51, no. 4, pp. 187–198, 1993.
View at: Google ScholarO Nusse, L Serrander, D P Lew, and K-H Krause, “-induced exocytosis in individual human neutrophils: high- and low-affinity granule populations and submaximal responses,” EMBO Journal, vol. 17, no. 5, pp. 1279–1288, 1998.
View at: Google ScholarO Nusse and M Lindau, “The calcium signal in human neutrophils and its relation to exocytosis investigated by patch-clamp capacitance and Fura-2 measurements,” Cell Calcium, vol. 14, no. 4, pp. 255–269, 1993.
View at: Google ScholarG Rothe and M Klouche, “Phagocyte function,” Methods in Cell Biology, vol. 75, pp. 679–708, 2004.
View at: Google ScholarA Böyum, “Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g,” Scandinavian Journal of Clinical and Laboratory Investigation, Supplement, vol. 97, pp. 77–89, 1968.
View at: Google ScholarO P Hamill, A Marty, E Neher, B Sakmann, and F J Sigworth, “Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches,” Pflugers Archiv, vol. 391, no. 2, pp. 85–100, 1981.
View at: Google ScholarM Lindau and E Neher, “Patch-clamp techniques for time-resolved capacitance measurements in single cells,” Pflugers Archiv, vol. 411, no. 2, pp. 137–146, 1988.
View at: Google ScholarE Neher and A Marty, “Discrete changes of cell membrane capacitance observed under conditions of enhanced secretion in bovine adrenal chromaffin cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 79, no. 21 I, pp. 6712–6716, 1982.
View at: Google ScholarC Joshi and J M Fernandez, “Capacitance measurements. An analysis of the phase detector technique used to study exocytosis and endocytosis,” Biophysical Journal, vol. 53, no. 6, pp. 885–892, 1988.
View at: Google ScholarD M Bers, C W Patton, and R Nuccitelli, “A practical guide to the preparation of buffers,” Methods in Cell Biology, vol. 40, pp. 3–29, 1994.
View at: Google ScholarN Borregaard, “Development of neutrophil granule diversity,” Annals of the New York Academy of Sciences, vol. 832, pp. 62–68, 1997.
View at: Google ScholarK Lollike and M Lindau, “Membrane capacitance techniques to monitor granule exocytosis in neutrophils,” Journal of Immunological Methods, vol. 232, no. 1-2, pp. 111–120, 1999.
View at: Google ScholarO Nusse and M Lindau, “The dynamics of exocytosis in human neutrophils,” Journal of Cell Biology, vol. 107, no. 6 I, pp. 2117–2123, 1988.
View at: Google ScholarH Sengelov, L Kjeldsen, and N Borregaard, “Control of exocytosis in early neutrophil activation,” Journal of Immunology, vol. 150, no. 4, pp. 1535–1543, 1993.
View at: Google ScholarA-S Cans, N Wittenberg, R Karlsson et al., “Artificial cells: unique insights into exocytosis using liposomes and lipid nanotubes,” Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 2, pp. 400–404, 2003.
View at: Google ScholarD Granfeldt and C Dahlgren, “An intact cytoskeleton is required for prolonged respiratory burst activity during neutrophil phagocytosis,” Inflammation, vol. 25, no. 3, pp. 165–169, 2001.
View at: Google ScholarK Lollike, M Lindau, J Calafat, and N Borregaard, “Compound exocytosis of granules in human neutrophils,” Journal of Leukocyte Biology, vol. 71, no. 6, pp. 973–980, 2002.
View at: Google ScholarA Karlsson and C Dahlgren, “Assembly and activation of the neutrophil NADPH oxidase in granule membranes,” Antioxidants and Redox Signaling, vol. 4, no. 1, pp. 49–60, 2002.
View at: Google Scholar