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Journal of Biomedicine and Biotechnology
Volume 2006 (2006), Article ID 97803, 7 pages
http://dx.doi.org/10.1155/JBB/2006/97803
Research Article

Neutrophil Secretion Induced by an Intracellular Ca2+ Rise and Followed by Whole-Cell Patch-Clamp Recordings Occurs Without any Selective Mobilization of Different Granule Populations

1The Phagocyte Research Laboratory, Department of Rheumatology and Inflammation Research, Göteborg University, Guldhedsgatan 10, Göteborg 413 46, Sweden
2Department of Chemistry & Bioscience, Chalmers University of Technology, Kemivägen 10, Göteborg 412 96, Sweden
3Univa AB, Lunds Universitet, Lund 221 00, Sweden

Received 20 December 2005; Revised 22 March 2006; Accepted 4 May 2006

Copyright © 2006 Daniel Granfeldt et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. N D Burg and M H Pillinger, “The neutrophil: function and regulation in innate and humoral immunity,” Clinical Immunology, vol. 99, no. 1, pp. 7–17, 2001. View at Publisher · View at Google Scholar
  2. G Berton, “Degranulation,” in Inflammation: Basic Principles and Clinical Correlates, J I Gallin and R Snyderman, Eds., pp. 703–719, Lippincott Williams & Wilkins, Philadelphia, Pa, 1999. View at Google Scholar
  3. N Borregaard and J B Cowland, “Granules of the human neutrophilic polymorphonuclear leukocyte,” Blood, vol. 89, no. 10, pp. 3503–3521, 1997. View at Google Scholar
  4. H Sengelov, P Follin, L Kjeldsen, K Lollike, C Dahlgren, and N Borregaard, “Mobilization of granules and secretory vesicles during in vivo exudation of human neutrophils,” Journal of Immunology, vol. 154, no. 8, pp. 4157–4165, 1995. View at Google Scholar
  5. T Sollner, S W Whiteheart, M Brunner et al., “SNAP receptors implicated in vesicle targeting and fusion,” Nature, vol. 362, no. 6418, pp. 318–324, 1993. View at Publisher · View at Google Scholar
  6. Y A Chen and R H Scheller, “SNARE-mediated membrane fusion,” Nature Reviews Molecular Cell Biology, vol. 2, no. 2, pp. 98–106, 2001. View at Publisher · View at Google Scholar
  7. J H Brumell, A Volchuk, H Sengelov et al., “Subcellular distribution of docking/fusion proteins in neutrophils, secretory cells with multiple exocytic compartments,” Journal of Immunology, vol. 155, no. 12, pp. 5750–5759, 1995. View at Google Scholar
  8. B Martin-Martin, S M Nabokina, J Blasi, P A Lazo, and F Mollinedo, “Involvement of SNAP-23 and syntaxin 6 in human neutrophil exocytosis,” Blood, vol. 96, no. 7, pp. 2574–2583, 2000. View at Google Scholar
  9. F Mollinedo, B Martin-Martin, J Calafat, S M Nabokina, and P A Lazo, “Role of vesicle-associated membrane protein-2, through Q-soluble N-ethylmaleimide-sensitive factor attachment protein receptor/R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor interaction, in the exocytosis of specific and tertiary granules of human neutrophils,” Journal of Immunology, vol. 170, no. 2, pp. 1034–1042, 2003. View at Google Scholar
  10. R D Burgoyne and A Morgan, “Ca2+ and secretory-vesicle dynamics,” Trends in Neurosciences, vol. 18, no. 4, pp. 191–196, 1995. View at Publisher · View at Google Scholar
  11. N W Andrews, “Regulated secretion of conventional lysosomes,” Trends in Cell Biology, vol. 10, no. 8, pp. 316–321, 2000. View at Publisher · View at Google Scholar
  12. P D Lew, A Monod, K-H Krause, F A Waldvogel, T J Biden, and W Schlegel, “The role of cytosolic free calcium in the generation of inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate in HL-60 cells. Differential effects of chemotactic peptide receptor stimulation at distinct Ca2+ levels,” Journal of Biological Chemistry, vol. 261, no. 28, pp. 13121–13127, 1986. View at Google Scholar
  13. M M Barrowman, S Cockcroft, and B D Gomperts, “Differential control of azurophilic and specific granule exocytosis in Sendai-virus-permeabilized rabbit neutrophils,” Journal of Physiology, vol. 383, pp. 115–124, 1987. View at Google Scholar
  14. N Borregaard, K Lollike, L Kjeldsen et al., “Human neutrophil granules and secretory vesicles,” European Journal of Haematology, vol. 51, no. 4, pp. 187–198, 1993. View at Google Scholar
  15. O Nusse, L Serrander, D P Lew, and K-H Krause, “Ca2+-induced exocytosis in individual human neutrophils: high- and low-affinity granule populations and submaximal responses,” EMBO Journal, vol. 17, no. 5, pp. 1279–1288, 1998. View at Publisher · View at Google Scholar
  16. O Nusse and M Lindau, “The calcium signal in human neutrophils and its relation to exocytosis investigated by patch-clamp capacitance and Fura-2 measurements,” Cell Calcium, vol. 14, no. 4, pp. 255–269, 1993. View at Publisher · View at Google Scholar
  17. G Rothe and M Klouche, “Phagocyte function,” Methods in Cell Biology, vol. 75, pp. 679–708, 2004. View at Google Scholar
  18. A Böyum, “Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g,” Scandinavian Journal of Clinical and Laboratory Investigation, Supplement, vol. 97, pp. 77–89, 1968. View at Google Scholar
  19. O P Hamill, A Marty, E Neher, B Sakmann, and F J Sigworth, “Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches,” Pflugers Archiv, vol. 391, no. 2, pp. 85–100, 1981. View at Publisher · View at Google Scholar
  20. M Lindau and E Neher, “Patch-clamp techniques for time-resolved capacitance measurements in single cells,” Pflugers Archiv, vol. 411, no. 2, pp. 137–146, 1988. View at Publisher · View at Google Scholar
  21. E Neher and A Marty, “Discrete changes of cell membrane capacitance observed under conditions of enhanced secretion in bovine adrenal chromaffin cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 79, no. 21 I, pp. 6712–6716, 1982. View at Publisher · View at Google Scholar
  22. C Joshi and J M Fernandez, “Capacitance measurements. An analysis of the phase detector technique used to study exocytosis and endocytosis,” Biophysical Journal, vol. 53, no. 6, pp. 885–892, 1988. View at Google Scholar
  23. D M Bers, C W Patton, and R Nuccitelli, “A practical guide to the preparation of Ca2+ buffers,” Methods in Cell Biology, vol. 40, pp. 3–29, 1994. View at Google Scholar
  24. N Borregaard, “Development of neutrophil granule diversity,” Annals of the New York Academy of Sciences, vol. 832, pp. 62–68, 1997. View at Publisher · View at Google Scholar
  25. K Lollike and M Lindau, “Membrane capacitance techniques to monitor granule exocytosis in neutrophils,” Journal of Immunological Methods, vol. 232, no. 1-2, pp. 111–120, 1999. View at Publisher · View at Google Scholar
  26. O Nusse and M Lindau, “The dynamics of exocytosis in human neutrophils,” Journal of Cell Biology, vol. 107, no. 6 I, pp. 2117–2123, 1988. View at Publisher · View at Google Scholar
  27. H Sengelov, L Kjeldsen, and N Borregaard, “Control of exocytosis in early neutrophil activation,” Journal of Immunology, vol. 150, no. 4, pp. 1535–1543, 1993. View at Google Scholar
  28. A-S Cans, N Wittenberg, R Karlsson et al., “Artificial cells: unique insights into exocytosis using liposomes and lipid nanotubes,” Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 2, pp. 400–404, 2003. View at Publisher · View at Google Scholar
  29. D Granfeldt and C Dahlgren, “An intact cytoskeleton is required for prolonged respiratory burst activity during neutrophil phagocytosis,” Inflammation, vol. 25, no. 3, pp. 165–169, 2001. View at Publisher · View at Google Scholar
  30. K Lollike, M Lindau, J Calafat, and N Borregaard, “Compound exocytosis of granules in human neutrophils,” Journal of Leukocyte Biology, vol. 71, no. 6, pp. 973–980, 2002. View at Google Scholar
  31. A Karlsson and C Dahlgren, “Assembly and activation of the neutrophil NADPH oxidase in granule membranes,” Antioxidants and Redox Signaling, vol. 4, no. 1, pp. 49–60, 2002. View at Publisher · View at Google Scholar