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Rafael Khatchadourian, Alexia Bachir, Samuel J. Clarke, Colin D. Heyes, Paul W. Wiseman, Jay L. Nadeau, "Fluorescence Intensity and Intermittency as Tools for Following Dopamine Bioconjugate Processing in Living Cells", BioMed Research International, vol. 2007, Article ID 070145, 10 pages, 2007. https://doi.org/10.1155/2007/70145
Fluorescence Intensity and Intermittency as Tools for Following Dopamine Bioconjugate Processing in Living Cells
CdSe/ZnS quantum dots (QDs) conjugated to biomolecules that quench their fluorescence, particularly dopamine, have particular spectral properties that allow determination of the number of conjugates per particle, namely, photoenhancement and photobleaching. In this work, we quantify these properties on a single-particle and ensemble basis in order to evaluate their usefulness as a tool for indicating QD uptake, breakdown, and processing in living cells. This creates a general framework for the use of fluorescence quenching and intermittency to better understand nanoparticle-cell interactions.
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Copyright © 2007 Rafael Khatchadourian et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.