Fluorescence Intensity and Intermittency as Tools for Following Dopamine Bioconjugate Processing in Living Cells

Khatchadourian, Rafael; Bachir, Alexia; Clarke, Samuel J.; Heyes, Colin D.; Wiseman, Paul W.; Nadeau, Jay L.

doi:https://doi.org/10.1155/2007/70145

BioMed Research International

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Biomedical Applications of Colloidal Nanocrystals

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Research Article | Open Access

Volume 2007 | Article ID 070145 | https://doi.org/10.1155/2007/70145

Fluorescence Intensity and Intermittency as Tools for Following Dopamine Bioconjugate Processing in Living Cells

Rafael Khatchadourian,¹Alexia Bachir,²Samuel J. Clarke,¹Colin D. Heyes,³Paul W. Wiseman,^2,3and Jay L. Nadeau¹

Academic Editor: Marek Osinski

Received10 Apr 2007

Revised11 Aug 2007

Accepted24 Dec 2007

Published24 Mar 2008

Abstract

CdSe/ZnS quantum dots (QDs) conjugated to biomolecules that quench their fluorescence, particularly dopamine, have particular spectral properties that allow determination of the number of conjugates per particle, namely, photoenhancement and photobleaching. In this work, we quantify these properties on a single-particle and ensemble basis in order to evaluate their usefulness as a tool for indicating QD uptake, breakdown, and processing in living cells. This creates a general framework for the use of fluorescence quenching and intermittency to better understand nanoparticle-cell interactions.

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Copyright

Copyright © 2007 Rafael Khatchadourian et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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