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Journal of Biomedicine and Biotechnology
Volume 2007 (2007), Article ID 71921, 9 pages
Research Article

Impact of Lactic Acid Bacteria on Dendritic Cells from Allergic Patients in an Experimental Model of Intestinal Epithelium

1Institut National de la Santé et de la Recherche Médicale (INSERM) U 416, Institut Fédératif de Recherche 17 (IFR 17), Institut Pasteur de Lille, Lille 59019, France
2Institut Pasteur de Lille, Lille 59019, France
3Université de Lille 2, Lille 59800, France
4Bactéries Lactiques et Immunité des Muqueuses, Institut Pasteur de Lille, Lille 59019, France
5The Department of Immunology, Faculty of Medicine, University of Manitoba, Basic Medical Sciences Building, Winnipeg, MB, Canada R3E 0W3

Received 10 October 2006; Revised 4 December 2006; Accepted 6 December 2006

Academic Editor: Abdelali Haoudi

Copyright © 2007 Céline Ratajczak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Lactic acid bacteria (LAB) are Gram positive nonpathogenic commensal organisms present in human gastrointestinal tract. In vivo, LAB are separated from antigen-presenting cells such as dendritic cells (DC) by the intestinal epithelial barrier. In this study, the impact of one LAB strain (Lactobacillus casei ATCC393) on human monocyte-derived DC from allergic and healthy donors was assessed by using a polarized epithelium model. Confocal and flow cytometer analyses showed that immature DC efficiently captured FITC-labelled L. casei through the epithelial layer. After interaction with L. casei, DC acquired a partial maturation status (i.e., CD86 and CD54 increase) and increased their interleukin (IL)-10 and IL-12 production. Interestingly, after activation by L. casei in the presence of experimental epithelium, DC from allergic patients instructed autologous naïve CD4+ T cells to produce more interferon-γ than without the epithelium. Thus by modulating human DC reactivity, LAB and intestinal epithelium might modify T cell immune response and regulate the development of allergic reaction.