Differential Effects of Leptin on the Invasive Potential of Androgen-Dependent and -Independent Prostate Carcinoma Cells
Figure 1
Cellular invasion and migration
of androgen-resistant prostate cancer cells are attenuated by leptin. PC-3 and
DU-145 cells were suspended in basal medium supplemented with 2% FBS, and 0.02% BSA, incubated with various
concentrations of leptin, and plated onto the upper surface of the Transwell
membrane inserts. For invasion studies, Matrigel was added to the upper surface
of the Transwell membrane and allowed to solidify in a sterile environment for
24 hours. After 10 hours of incubation at in a incubator,
cellular invasion (a) and migration (b) were measured
by counting the cells that had migrated to the lower surface of the membrane.
Values represent the mean ±S.E. of triplicate samples of a representative
experiment.
