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Journal of Biomedicine and Biotechnology
Volume 2008 (2008), Article ID 394802, 6 pages
http://dx.doi.org/10.1155/2008/394802
Research Article

Jaceosidin Induces Apoptosis in Human Ovary Cancer Cells through Mitochondrial Pathway

1The Second Affiliated Hospital, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China
2State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China

Received 14 January 2008; Accepted 6 July 2008

Academic Editor: Wolfgang Schulz

Copyright © 2008 Wen Lv et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We examined the antiproliferation effect of Jaceosidin ( , 5, 7-trihydroxy- , 6-dimethoxyflavone) isolated from the herb of Artemisia vestita Wall on several human cancer cell lines. Jaceosidin significantly reduced the proliferation of CAOV-3, SKOV-3, HeLa, and PC3 cells in a concentration-dependent manner. A time-dependent inhibition was also observed in CAOV-3 cells by Jaceosidin. By flow cytometric analysis, we found that Jaceosidin treatment resulted in an increased apoptosis in CAOV-3 cells. The cells treated with Jaceosidin exhibited a decreased mitochondrial membrane potential. Jaceosidin also increased the level of cleaved caspase-9 and induced the cleavage of caspase-3 and poly (ADP-ribose) polymerase (PARP), while caspase-3 inhibitor Z-DEVD-FMK significantly reversed the proapoptotic effect of Jaceosidin in CAOV-3 cells. Moreover, Jaceosidin elevated the level of cytochrome c in cytosol. These findings suggest that the anticancer effect of Jaceosidin may be contributed by an induction of apoptosis involving cytochrome c release from mitochondria to cytosol.