Jaceosidin Induces Apoptosis in Human Ovary Cancer Cells through Mitochondrial Pathway
Figure 3
Jaceosidin
dose-dependently (a) induces loss of mitochondrial membrane potential and (b) induces
cytochrome c to release from
mitochondria to cytosol. CAOV-3
cells were seeded into 12-well-plate and cultured overnight. Jaceosidin or
quercetin was added into each well. Twenty-four hours later, cells were
harvested for measuring mitochondrial membrane potential by flow cytometry
using JC-1 staining. Cells on the lower side showed lower membrane potential. One
of three independent experiments is shown. In addition, mitochondria and
cytosol proteins were prepared from cells treated with 80 M jaceosidin,
which were subjected to western blotting for measuring cytochrome c in mitochondria and cytosol.
